Diversity Outbred Mice: Maintaining Variation

Diversity Outbred Mice: Maintaining Variation

Description 

We detected strong transmission ratio distortion in the Diversity Outbred (DO) mouse population on chromosome 2, caused by meiotic drive favoring transmission of the WSB/EiJ allele at the R2d2 locus. The distorted region harbors thousands of polymorphisms derived from the seven non-WSB founder strains and many of these would be lost if the sweep was allowed to continue. To ensure the utility of the DO population to study genetic variation on chromosome 2, we performed an artificial selection against WSB/EiJ alleles at the R2d2 locus. We have sucessfully purged theWSB/EiJ allele from the drive locus while preserving WSB/EiJ alleles in the flanking regions.

 

Data

We provide genotyping data - allele calls and probe intensities - spanning generations G6 - G21 of the DO as zipped (.tar.gz) files:

Publication 

Chesler EJ, Gatti DM, Morgan AP, Strobel M, Trepanier L, Oberbeck D, McWeeney S, Hitzemann R, Ferris M, McMullan R, Clayshultle A, Bell TA, Pardo-Manuel de Villena F, Churchill GA
G3