Dynamic gene expression changes during sleep in Drosophila brain

Gene expression in the brain of Drosophila melanogaster was measured using GeneChip Drosophila microarrays (Affymetrix) after different durations of sleep and periods of sleep deprivation. There were a total of ten time groups, with three biological replicates. Seven time groups were used to determine differential gene expression between normal and sleep-deprived flies, whereas three time groups were used to test for differential gene expression due to the effect of mechanical stimulation. For further details, see Zimmerman et al., 2006.